IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
![IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25369/GTX25369_1278_IHC-P_w_23060722_204.webp "IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20")
![WB analysis of whole cell extracts (20 microg lysate) of COS-7 (Lane 1), MCF7 (Lane 2), PC-12 (Lane 3), HeLa (lane 4), Jurkat (lane 5), NIH/3T3 (lane 6) and A-431 (lane 7) using GTX25369 eIF2 alpha antibody [EIF2a]. Dilution : 1:500-1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25369/GTX25369_1767_WB_w_23060722_764.webp "WB analysis of whole cell extracts (20 microg lysate) of COS-7 (Lane 1), MCF7 (Lane 2), PC-12 (Lane 3), HeLa (lane 4), Jurkat (lane 5), NIH/3T3 (lane 6) and A-431 (lane 7) using GTX25369 eIF2 alpha antibody [EIF2a]. Dilution : 1:500-1:1000")
![ICC/IF analysis of A549 cells using GTX25369 eIF2 alpha antibody [EIF2a]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton X-100 for 10 minutes Dilution : 1:250 dilution in1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25369/GTX25369_636_ICC-IF_w_23060722_337.webp "ICC/IF analysis of A549 cells using GTX25369 eIF2 alpha antibody [EIF2a]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton X-100 for 10 minutes Dilution : 1:250 dilution in1% BSA and incubated for 3 hours at room temperature")
IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
eIF2 alpha antibody [EIF2a]
GTX25369
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rat
TargetEIF2S1
Overview
- SupplierGeneTex
- Product NameeIF2 alpha antibody [EIF2a]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2A).
- Application Supplier NoteWB: 1:500-1:1000. ICC/IF: 1:250. IHC-P: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEIF2a
- ConjugateUnconjugated
- Gene ID1965
- Target nameEIF2S1
- Target descriptioneukaryotic translation initiation factor 2 subunit alpha
- Target synonymsEIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- HostMouse
- IsotypeIgG1
- Protein IDP05198
- Protein NameEukaryotic translation initiation factor 2 subunit 1
- Scientific DescriptionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
- ReactivityHuman, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Wei PC et al., 2012 Jan, Nucleic Acids ResRead more