More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Hec1 (non-phospho Ser 165) antibody
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (non-phospho Ser 165) antibody (GTX70012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (non-phospho Ser 165) antibody (GTX70012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (non-phospho Ser 165) antibody (GTX70012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Hec1 (non-phospho Ser 165) antibody

GTX70012
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDC80
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Hec1 (non-phospho Ser 165) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Recommended Starting Dilutions:For WB: Use at a dilution of 1:600For ICC/IF: Use at a dilution of 1:300Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.91 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10403
  • Target name
    NDC80
  • Target description
    NDC80 kinetochore complex component
  • Target synonyms
    HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, kinetochore protein NDC80 homolog, NDC80 homolog, kinetochore complex component, NDC80 kinetochore complex component homolog, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, kinetochore associated 2, kinetochore protein Hec1, kinetochore-associated protein 2, retinoblastoma-associated protein HEC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14777
  • Protein Name
    Kinetochore protein NDC80 homolog
  • Scientific Description
    This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Kinetochore protein NDC80 homolog [Hec1 (9G3)]AB03500-1.1-BT
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
ReactivityHuman
TargetNDC80
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NDC80 Antibody144-05411
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetNDC80
  • SizePrice
Figure 1. Western blot analysis of NDC80 using anti-NDC80 antibody (A01731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDC80 antigen affinity purified polyclonal antibody (Catalog # A01731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDC80 at approximately 74KD. The expected band size for NDC80 is at 74KD.
Product group Antibodies
Boster Bio
Anti-HEC1/HEC/NDC80 Antibody Picoband(r)A01731-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetNDC80
  • SizePrice
Sample A: 30ug of A431 whole cell lysate (GTX27909) B: 30ug of H1299 whole cell lysate 7.5% SDS PAGE GTX110735 diluted at 1:1000
Product group Antibodies
References
GeneTex
Hec1 antibodyGTX110735
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetNDC80
  • SizePrice
WB analysis of (1) Jurkat, (2) HeLa, (3) NCCIT and (4) human spleen lysates (10 μg per lane) using HEC1 antibody [EPR5342] at a dilution of 1:10,000.
Product group Antibodies
GeneTex
Hec1 antibody [EPR5342]GTX63082
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetNDC80
  • SizePrice
Western Blot analysis of phospho and non-phospho Ser76/Ser77
Product group Antibodies
GeneTex
Hec1 (non-phospho Ser 76/77) antibodyGTX70014
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetNDC80
  • SizePrice
Western Blot analysis of phospho and non-phospho Ser76/Ser77
Product group Antibodies
GeneTex
Hec1 (phospho Ser 76/77) antibodyGTX70015
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetNDC80
  • SizePrice
Immunofluorescent staining of Hec1 non-phospho Ser55 in cells undergoing prometaphase (top) or metaphase (bottom)
Product group Antibodies
GeneTex
Hec1 antibodyGTX70016
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetNDC80
  • SizePrice
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (phospho Ser 55) antibody (GTX70017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
Hec1 (phospho Ser 55) antibodyGTX70017
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetNDC80
  • SizePrice
Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
References
GeneTex
Hec1 antibody [9G3.23]GTX70268
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
ReactivityHamster, Human, Mammals, Mouse
TargetNDC80
  • SizePrice