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ProductsBack/Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]

GTX628789
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetH2AX
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT2311
  • Concentration
    0.92 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3014
  • Target name
    H2AX
  • Target description
    H2A.X variant histone
  • Target synonyms
    H2A.X, H2A/X, H2AFX, histone H2AX, H2A histone family member X, H2AX histone, histone H2A.x
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16104
  • Protein Name
    Histone H2AX
  • Scientific Description
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yu LS, Jhunjhunwala M, Hong SY, et al. Tissue Architecture Influences the Biological Effectiveness of Boron Neutron Capture Therapy in In Vitro/In Silico Three-Dimensional Self-Assembly Cell Models of Pancreatic Cancers. Cancers (Basel). 2021,13(16). doi: 10.3390/cancers13164058
    Read this paper
  • Tomita T, Kato M, Mishima T, et al. Extracellular mRNA transported to the nucleus exerts translation-independent function. Nat Commun. 2021,12(1):3655. doi: 10.1038/s41467-021-23969-1
    Read this paper
  • Chen TY, Huang BM, Tang TK, et al. Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response. Cell Death Differ. 2021,28(6):1865-1879. doi: 10.1038/s41418-020-00713-8
    Read this paper
  • Li G, Chen S, Zhang Y, et al. Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage. J Cell Sci. 2021,134(2):pii: jcs243394. doi: 10.1242/jcs.243394.
    Read this paper
  • Lin BZ, Wan SY, Lin MY, et al. Involvement of Differentially Expressed microRNAs in the PEGylated Liposome Encapsulated (188)Rhenium-Mediated Suppression of Orthotopic Hypopharyngeal Tumor. Molecules. 2020,25(16). doi: 10.3390/molecules25163609
    Read this paper
  • Honda T, Inui M. PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression. Sci Rep. 2020,10(1):1140. doi: 10.1038/s41598-020-58116-1
    Read this paper
  • Wang CY, Chang CY, et al. N-Dihydrogalactochitosan Potentiates the Radiosensitivity of Liver Metastatic Tumor Cells Originated from Murine Breast Tumors. Int J Mol Sci. 2019,20(22). doi: 10.3390/ijms20225581
    Read this paper
  • Lin ST, Ma CC, Kuo KT, et al. Transcription Factor Elf3 Modulates Vasopressin-Induced Aquaporin-2 Gene Expression in Kidney Collecting Duct Cells. Front Physiol. 2019,10:1308. doi: 10.3389/fphys.2019.01308
    Read this paper
  • Tamanaha-Nakasone A, Uehara K, Tanabe Y, et al. K1 gene transformation activities in AIDS-related and classic type Kaposi's sarcoma: Correlation with clinical presentation. Sci Rep. 2019,9(1):6416. doi: 10.1038/s41598-019-42763-0
    Read this paper
  • Kong X, Cruz GMS, Trinh SL, et al. Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair. J Cell Sci. 2018,131(23). doi: 10.1242/jcs.219311
    Read this paper

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