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ProductsBack/Histone H2A.XS139ph (phospho Ser139) antibody
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract (untreated) B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract (untreated) B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract (untreated) B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H2A.XS139ph (phospho Ser139) antibody

GTX127340
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetH2AX
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H2A.XS139ph (phospho Ser139) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.16 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3014
  • Target name
    H2AX
  • Target description
    H2A.X variant histone
  • Target synonyms
    H2A.X, H2A/X, H2AFX, histone H2AX, H2A histone family member X, H2AX histone, histone H2A.x
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16104
  • Protein Name
    Histone H2AX
  • Scientific Description
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Giang LH, Wu KS, Lee WC, et al. Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor. J Exp Clin Cancer Res. 2023,42(1):346. doi: 10.1186/s13046-023-02911-x
    Read this paper
  • Jeong H, Wie M, Baek IJ, et al. TRIP13 Participates in Immediate-Early Sensing of DNA Strand Breaks and ATM Signaling Amplification through MRE11. Cells. 2022,11(24). doi: 10.3390/cells11244095
    Read this paper
  • Zhou X, Zhou Q, Zhou D, et al. MicroRNA expression profiles in extracellular vesicles and intracellular of AURKA inhibitor-induced senescent neuroblastoma cells. Transl Cancer Res. 2022,11(8):2767-2782. doi: 10.21037/tcr-21-2438
    Read this paper
  • Shih HT, Chen WY, Wang HY, et al. DNMT3b protects centromere integrity by restricting R-loop-mediated DNA damage. Cell Death Dis. 2022,13(6):546. doi: 10.1038/s41419-022-04989-1
    Read this paper
  • Huang S, Wang X, Lin G, et al. Discovery of human TyrRS inhibitors by structure-based virtual screening, structural optimization, and bioassays. RSC Adv. 2019,9(16):9323-9330. doi: 10.1039/c9ra00458k
    Read this paper
  • Yang JL, Yang MD, Chen JC, et al. Ouabain Induces DNA Damage in Human Osteosarcoma U-2 OS Cells and Alters the Expression of DNA Damage and DNA Repair-associated Proteins. In Vivo. 2021,35(5):2687-2696. doi: 10.21873/invivo.12552
    Read this paper
  • Vanhunsel S, Bergmans S, Beckers A, et al. The killifish visual system as an in vivo model to study brain aging and rejuvenation. NPJ Aging Mech Dis. 2021,7(1):22. doi: 10.1038/s41514-021-00077-4
    Read this paper
  • Cheng YC, Chen PY, Way TD, et al. Pre-Treatment of Pterostilbene Enhances H(2)O(2)-induced Cell Apoptosis Through Caspase-dependent Pathway in Human Keratinocyte Cells. In Vivo. 2021,35(2):833-843. doi: 10.21873/invivo.12324
    Read this paper
  • Zhao T, Ye S, Tang Z, et al. Loss-of-function of p53 isoform Δ113p53 accelerates brain aging in zebrafish. Cell Death Dis. 2021,12(2):151. doi: 10.1038/s41419-021-03438-9
    Read this paper
  • Chao T, Shih HT, Hsu SC, et al. Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability. Autophagy. 2021,17(11):3444-3460. doi: 10.1080/15548627.2021.1874209
    Read this paper

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Figure 1. Western blot analysis of H2AFX using anti-H2AFX antibody (A00241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFX antigen affinity purified polyclonal antibody (Catalog # A00241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.
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Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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