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ProductsBack/Iba1 antibody [HL22]
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.

Iba1 antibody [HL22]

GTX635363
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat
TargetAIF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Iba1 antibody [HL22]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL22
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor 1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Scientific Description
    This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
  • Reactivity
    Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Iacono D, Murphy EK, Stimpson CD, et al. Low-dose brain radiation: lowering hyperphosphorylated-tau without increasing DNA damage or oncogenic activation. Sci Rep. 2023,13(1):21142. doi: 10.1038/s41598-023-48146-w
    Read this paper
  • Brown TC, Crouse EC, Attaway CA, et al. Microglia are dispensable for experience-dependent refinement of visual circuitry. bioRxiv. 2023,:pii: 2023.10.17.562708. doi: 10.1101/2023.10.17.562708.
    Read this paper
  • Wang HK, Su YT, Ho YC, et al. HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis. J Inflamm Res. 2023,16:4103-4116. doi: 10.2147/JIR.S416239
    Read this paper
  • Wong CE, Hu CY, Lee PH, et al. Sciatic nerve stimulation alleviates acute neuropathic pain via modulation of neuroinflammation and descending pain inhibition in a rodent model. J Neuroinflammation. 2022,19(1):153. doi: 10.1186/s12974-022-02513-y
    Read this paper
  • Fan Z, Zhang W, Cao Q, et al. JAK2/STAT3 pathway regulates microglia polarization involved in hippocampal inflammatory damage due to acute paraquat exposure. Ecotoxicol Environ Saf. 2022,234:113372. doi: 10.1016/j.ecoenv.2022.113372
    Read this paper
  • Zhang Y, Hou B, Liang P, et al. TRPV1 channel mediates NLRP3 inflammasome-dependent neuroinflammation in microglia. Cell Death Dis. 2021,12(12):1159. doi: 10.1038/s41419-021-04450-9
    Read this paper
  • Yan H, Zhao H, Kang Y, et al. Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition. Neuropharmacology. 2021,194:108627. doi: 10.1016/j.neuropharm.2021.108627
    Read this paper
  • Xiong LL, Tan YX, Du RL, et al. Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action. Am J Chin Med. 2021,49(3):677-703. doi: 10.1142/S0192415X21500312
    Read this paper
  • Teng SW, Sung HY, Wen YC, et al. Potential surrogate quantitative immunomodulatory potency assay for monitoring human umbilical cord-derived mesenchymal stem cells production. Cell Biol Int. 2021,45(5):1072-1081. doi: 10.1002/cbin.11553
    Read this paper

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Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
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