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ProductsBack/IL2 Mouse Monoclonal Antibody [Clone ID: 8D5]
Western blot analysis using IL2 mouse mAb against full-length IL2 recombinant protein with Trx tag (1) and full-length IL2-hIgGFc transfected HEK293 cell lysate (2).
Western blot analysis using IL2 mouse mAb against full-length IL2 recombinant protein with Trx tag (1) and full-length IL2-hIgGFc transfected HEK293 cell lysate (2).
Western blot analysis using IL2 mouse mAb against full-length IL2 recombinant protein with Trx tag (1) and full-length IL2-hIgGFc transfected HEK293 cell lysate (2).

IL2 Mouse Monoclonal Antibody [Clone ID: 8D5]

AM06310SU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetIL2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    IL2 Mouse Monoclonal Antibody [Clone ID: 8D5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8D5
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    IL-2, TCGF, lymphokine, interleukin-2, T cell growth factor, aldesleukin, involved in regulation of T-cell clonal expansion
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP60568
  • Protein Name
    Interleukin-2
  • Scientific Description
    IL2 mouse monoclonal antibody, clone 8D5, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
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