![WB analysis of various samples using GTX08968 KAP1 antibody [GT1161]. Dilution : 1:1000 Loading : 25 μg WB analysis of various samples using GTX08968 KAP1 antibody [GT1161]. Dilution : 1:1000 Loading : 25 μg](https://www.genetex.com/upload/website/prouct_img/normal/GTX08968/GTX08968_20200508_WB_w_23053123_747.webp)
WB analysis of various samples using GTX08968 KAP1 antibody [GT1161]. Dilution : 1:1000 Loading : 25 μg
KAP1 antibody [GT1161]
GTX08968
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRIM28
Overview
- SupplierGeneTex
- Product NameKAP1 antibody [GT1161]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1161
- ConjugateUnconjugated
- Gene ID10155
- Target nameTRIM28
- Target descriptiontripartite motif containing 28
- Target synonymsKAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta, WT7, transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, KAP-1, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcriptional intermediary factor 1-beta
- HostRabbit
- IsotypeIgG
- Protein IDQ13263
- Protein NameTranscription intermediary factor 1-beta
- Scientific DescriptionThe protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of mouse brain tissue section using GTX08968 KAP1 antibody [GT1161]. Dlution : 1:100 IHC-P analysis of mouse brain tissue section using GTX08968 KAP1 antibody [GT1161]. Dlution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX08968/GTX08968_20191101_AP_003_78_w_23053123_785.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [GT1161] (GTX08968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [GT1161] (GTX08968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX08968/GTX08968_4000000047_20200410_WB_w_23053123_520.webp)
![IHC-P analysis of rat spleen tissue section using GTX08968 KAP1 antibody [GT1161]. Dlution : 1:100 IHC-P analysis of rat spleen tissue section using GTX08968 KAP1 antibody [GT1161]. Dlution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX08968/GTX08968_20191101_AP_003_76_w_23053123_862.webp)
![IHC-P analysis of hmuan appendix tissue section using GTX08968 KAP1 antibody [GT1161]. Dlution : 1:100 IHC-P analysis of hmuan appendix tissue section using GTX08968 KAP1 antibody [GT1161]. Dlution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX08968/GTX08968_20191101_AP_003_77_w_23053123_751.webp)
![IP analysis of HeLa cell lysate using GTX08968 KAP1 antibody [GT1161]. Antibody amount : 3μg / 300μg lysate Dilution : 1:1000 IP analysis of HeLa cell lysate using GTX08968 KAP1 antibody [GT1161]. Antibody amount : 3μg / 300μg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX08968/GTX08968_20210323_IP_8_w_23053123_745.webp)



![KAP1 antibody [HL1536] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1536] (GTX637010) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637010/GTX637010_T-44690_20220701_ICC_IF_22071401_907.webp)
![KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637013/GTX637013_T-44690_20220610_IHC-P_R_22062121_492.webp)
![WB analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX80695/GTX80695_1955_WB_w_23061322_161.webp)

