KAP1 antibody [HL1536] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1536] (GTX637010) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1536] (GTX637010) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637010/GTX637010_44739_20220715_WB_22072519_502.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1536] (GTX637010) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
KAP1 antibody [HL1536] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1536] (GTX637010) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
KAP1 antibody [HL1536]
GTX637010
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM28
Overview
- SupplierGeneTex
- Product NameKAP1 antibody [HL1536]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1536
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10155
- Target nameTRIM28
- Target descriptiontripartite motif containing 28
- Target synonymsKAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta, WT7, transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, KAP-1, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-interacting protein 1, KRIP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, TIF1-beta, nuclear corepressor KAP-1, protein phosphatase 1, regulatory subunit 157, transcriptional intermediary factor 1-beta
- HostRabbit
- IsotypeIgG
- Protein IDQ13263
- Protein NameTranscription intermediary factor 1-beta
- Scientific DescriptionThe protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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