FACS analysis of HeLa cells using GTX01140 MLH1 antibody [SP08-04]. Red : primary antibody Black : unlabelled control Dilution : 1:50
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody [SP08-04] (GTX01140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01140/GTX01140_HM0812_20200228_WB_w_23053121_521.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MLH1 antibody [SP08-04] (GTX01140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
FACS analysis of HeLa cells using GTX01140 MLH1 antibody [SP08-04]. Red : primary antibody Black : unlabelled control Dilution : 1:50
MLH1 antibody [SP08-04] - Recombinant antibody Validated
GTX01140
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetMLH1
Overview
- SupplierGeneTex
- Product NameMLH1 antibody [SP08-04] - Recombinant antibody Validated
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSP08-04
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4292
- Target nameMLH1
- Target descriptionmutL homolog 1
- Target synonymsCOCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1; mutL homolog 1, colon cancer, nonpolyposis type 2
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThe protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203