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ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00973 MLKL (phospho Ser358) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml

MLKL (phospho Ser358) antibody

GTX00973
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMLKL
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Overview

  • Supplier
    GeneTex
  • Product Name
    MLKL (phospho Ser358) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID197259
  • Target name
    MLKL
  • Target description
    mixed lineage kinase domain like pseudokinase
  • Target synonyms
    hMLKL, mixed lineage kinase domain-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB16
  • Protein Name
    Mixed lineage kinase domain-like protein
  • Scientific Description
    This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Low-frequency pulsed electromagnetic fields alleviate the condylar cartilage degeneration and synovitis at the early stage of temporomandibular joint osteoarthritis.
    Read more
  • Triclosan-induced neuroinflammation develops caspase-independent and TNF-alpha signaling pathway associated necroptosis in Neuro-2a cells. Katiyar P et al., 2022, Curr Res Toxicol
    Read more

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Figure 1. Western blot analysis of MLKL using anti-MLKL antibody (A00535-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL antigen affinity purified polyclonal antibody (Catalog # A00535-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLKL at approximately 50 kDa. The expected band size for MLKL is at 54 kDa.
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