IHC-P analysis of human breast tissue using GTX19348 N-Cadherin antibody [8C11]. A : isotype control B : Primary antibody C : Primary antibody with magnified section Antigen retrieval : HEIR with a buffer (pH 6.2) Dilution : 2 microg/ml
![IHC-P analysis of human heart tissue using GTX19348 N-Cadherin antibody [8C11]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX19348/GTX19348_1069_IHC-P_w_23060620_202.webp "IHC-P analysis of human heart tissue using GTX19348 N-Cadherin antibody [8C11]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20")
![IP analysis of SH-SY5Y cell lysates using GTX19348 N-Cadherin antibody [8C11]. GTX19348 was used as IP and IB antibody IP reaction : 5microg antibody / 300microg lysate WB antibody dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX19348/GTX19348_1431_IP_w_23060620_760.webp "IP analysis of SH-SY5Y cell lysates using GTX19348 N-Cadherin antibody [8C11]. GTX19348 was used as IP and IB antibody IP reaction : 5microg antibody / 300microg lysate WB antibody dilution : 1:1000")
![WB analysis of 25 ug of Hela (Lane 1), HUVEC (Lane 2), and C2C12 cell lysates (Lane 3) using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000 (Hela) and 1:500 (HUVEC and C2C12)](https://www.genetex.com/upload/website/prouct_img/normal/GTX19348/GTX19348_1566_WB_w_23060620_784.webp "WB analysis of 25 ug of Hela (Lane 1), HUVEC (Lane 2), and C2C12 cell lysates (Lane 3) using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000 (Hela) and 1:500 (HUVEC and C2C12)")
![WB analysis of 25 microg of various lysates using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX19348/GTX19348_1567_WB_w_23060620_176.webp "WB analysis of 25 microg of various lysates using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000")
![WB analysis of tissue lysate and whole cell extract (30 ug) of Mouse Brain (Lane 1) and MCF-7 (Lane 2) using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX19348/GTX19348_1568_WB_w_23060620_528.webp "WB analysis of tissue lysate and whole cell extract (30 ug) of Mouse Brain (Lane 1) and MCF-7 (Lane 2) using GTX19348 N-Cadherin antibody [8C11]. Dilution : 1:1000")
![ICC/IF analysis of SH-SY5Y cells using GTX19348 N-Cadherin antibody [8C11]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX19348/GTX19348_364_ICC-IF_w_23060620_566.webp "ICC/IF analysis of SH-SY5Y cells using GTX19348 N-Cadherin antibody [8C11]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature")
IHC-P analysis of human breast tissue using GTX19348 N-Cadherin antibody [8C11]. A : isotype control B : Primary antibody C : Primary antibody with magnified section Antigen retrieval : HEIR with a buffer (pH 6.2) Dilution : 2 microg/ml
N-Cadherin antibody [8C11]
GTX19348
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAvian, Hamster, Human, Mouse, Rabbit
TargetCDH2
Overview
- SupplierGeneTex
- Product NameN-Cadherin antibody [8C11]
- Delivery Days Customer9
- Antibody SpecificityThis antibody is not suitable for frozen tissues in immunohistochemistry applications.
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:50-1:200. IHC-P: 1:20. IP: 5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8C11
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID1000
- Target nameCDH2
- Target descriptioncadherin 2
- Target synonymsACOGS; ARVD14; cadherin 2, type 1, N-cadherin (neuronal); cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDHN; CDw325; NCAD; N-cadherin 1; neural cadherin
- HostMouse
- IsotypeIgG1
- Protein IDP19022
- Protein NameCadherin-2
- Scientific DescriptionThis gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
- ReactivityAvian, Hamster, Human, Mouse, Rabbit
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203