
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
N-Cadherin antibody
GTX127345
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCDH2
Overview
- SupplierGeneTex
- Product NameN-Cadherin antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.15 mg/ml
- ConjugateUnconjugated
- Gene ID1000
- Target nameCDH2
- Target descriptioncadherin 2
- Target synonymsACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD, cadherin-2, N-cadherin 1, cadherin 2, type 1, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural cadherin
- HostRabbit
- IsotypeIgG
- Protein IDP19022
- Protein NameCadherin-2
- Scientific DescriptionThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chiou WC, Lyu YS, Hsia TL, et al. Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor. Biomed Pharmacother. 2024,170:116077. doi: 10.1016/j.biopha.2023.116077Read this paper
- Adamiok-Ostrowska A, Grzanka M, Czarnocka B. Agrin is a novel oncogenic protein in thyroid cancer. Oncol Lett. 2023,26(5):483. doi: 10.3892/ol.2023.14070Read this paper
- Yin L, Ye Y, Zou L, et al. AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. J Exp Clin Cancer Res. 2023,42(1):204. doi: 10.1186/s13046-023-02776-0Read this paper
- Hung TH, Huang Y, Yeh CT, et al. High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma. Biomed J. 2024,47(2):100612. doi: 10.1016/j.bj.2023.100612Read this paper
- Chiou HC, Wang CW, Chen SC, et al. Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human. Antioxidants (Basel). 2023,12(2). doi: 10.3390/antiox12020532Read this paper
- Chang KS, Chen ST, Sung HC, et al. WNT1 Inducible Signaling Pathway Protein 1 Is a Stroma-Specific Secreting Protein Inducing a Fibroblast Contraction and Carcinoma Cell Growth in the Human Prostate. Int J Mol Sci. 2022,23(19). doi: 10.3390/ijms231911437Read this paper
- Kosim MY, Fukazawa T, Miyauchi M, et al. p53 status modifies cytotoxic activity of lactoferrin under hypoxic conditions. Front Pharmacol. 2022,13:988335. doi: 10.3389/fphar.2022.988335Read this paper
- Chua HH, Chang MH, Chen YH, et al. PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis. Cell Mol Gastroenterol Hepatol. 2023,15(1):121-152. doi: 10.1016/j.jcmgh.2022.09.014Read this paper
- Martínez Traverso IM, Steimle JD, Zhao X, et al. LATS1/2 control TGFB-directed epithelial-to-mesenchymal transition in the murine dorsal cranial neuroepithelium through YAP regulation. Development. 2022,149(18). doi: 10.1242/dev.200860Read this paper
- Lee WJ, Cheng TC, Yen Y, et al. Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects. J Food Drug Anal. 2021,29(1):113-127. doi: 10.38212/2224-6614.3230Read this paper




![N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX127345/GTX127345_40975_20160921_IHC-Fr_w_23060522_431.webp)







![Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (GTX112733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112733/GTX112733_40394_20180810_WB_KO_watermark_w_23060500_602.webp)
![N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112734/GTX112734_40394_20180704_ICC_IF_w_23060500_124.webp)
![IHC-P analysis of human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D) using GTX82992 N-Cadherin antibody [5D5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX82992/GTX82992_20170912_IHC-P_w_23061322_220.webp)