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Primary Antibodies

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Western Blot of Rabbit anti-IL-9 Antibody (HRP).Load: 50 ng Human IL-9 per lane. IL-9 Antibody (HRP) at 1:1,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 14 kDa
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL9
  • SizePrice
GeneTex's anti-Human IL-9 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-9. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
IL9 antibodyGTX48672
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL9
  • SizePrice
Anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax? chemiluminescent substrate.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL7
  • SizePrice
Anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.
Product group Antibodies
GeneTex
ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL7
  • SizePrice
GeneTex's anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
IL7 antibodyGTX48675
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL7
  • SizePrice
Anti-human IL-29 antibody in western blot shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 μg, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human IL-29 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIFNL1
  • SizePrice
Western blot using GeneTex's affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1.
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetRSL1D1
  • SizePrice
Western Blot of Biotin Conjugated Rabbit anti-IL-27/p28 antibody. Load: 50 ng Mouse IL-27/p28 per lane. Blocking for 30 min at RT. Predicted/Observed size: 24 kDa for Mouse IL-27/p28. Other band(s): None.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIl27
  • SizePrice
Recombinant mouse IL27/p28 was loaded at 0.25 μg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with rabbit anti-Mouse IL-27/p28 (HRP) antibody in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDocR 4000 MP.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIl27
  • SizePrice
Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody (GTX48686). Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 μg and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4oC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetIl27
  • SizePrice
PARC antibody
Product group Antibodies
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCUL9
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCcl20
  • SizePrice

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