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Primary Antibodies

469 to 480 of 39.385 products
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Product group Antibodies
GeneTex
MDC1 antibodyGTX48652
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMDC1
  • SizePrice
Western blot using GeneTex's HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
Product group Antibodies
GeneTex
IL32A antibody (Biotin)GTX48653
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL32
  • SizePrice
Western blot using GeneTex's HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
Product group Antibodies
GeneTex
IL32A antibody (HRP)GTX48654
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL32
  • SizePrice
Western blot using GeneTex's affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4o C.
Product group Antibodies
GeneTex
MFAP5 antibodyGTX48656
ApplicationsWestern Blot, ELISA
ReactivityHuman, Primate
TargetMFAP5
  • SizePrice
Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.
Product group Antibodies
References
GeneTex
TAF1 antibodyGTX48659
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetTAF1
  • SizePrice
Western Blot of Anti-human MCP-1 antibody shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 μg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human MCP-1 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
Product group Antibodies
GeneTex
MCP1 / CCL2 antibodyGTX48661
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCCL2
  • SizePrice
GeneTex's anti-Cyclin L1a antibody was used at a 10 μg/ml to detect Cyclin L1a in a variety of tissues including breast (collagen), heart, kidney (distal tubules), liver, skeletal muscle, ovary μgranulosa and oocyte), pancreas (islet), placenta (trophoblast), prostate (epithelium), skin, spleen (endothelium), stomach (chief), testes (seminiferous epithelium and leydig), thymus (Has-sal's corpuscle and lymphocytes) and uterus μglandular epithelium and stroma). Low to moderate background staining was noted. This image shows Cyclin L1a staining of human spleen. Tissue was formalin-fixed and paraffin embedded.
Product group Antibodies
References
GeneTex
Cyclin L1 (Isoform 1) antibodyGTX48663
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityDrosophila, Human, Mouse
TargetCCNL1
  • SizePrice
CAND1 antibody
Product group Antibodies
GeneTex
CAND1 antibodyGTX48664
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCAND1
  • SizePrice
Ube2J1 antibody
Product group Antibodies
GeneTex
Ube2J1 antibodyGTX48665
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
ReactivityHuman
TargetUBE2J1
  • SizePrice
GeneTex's anti-NF-Y (A Subunit) antibody was diluted at 10 μg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.
Product group Antibodies
GeneTex
NFYA antibodyGTX48667
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman
TargetNFYA
  • SizePrice
Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. GeneTex's Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using GeneTex's anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4oC. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
ATF3 antibodyGTX48669
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetATF3
  • SizePrice
Western Blot of Rabbit anti-Human IL-9 Biotin Conjugated Antibody. Load: 50 ng Human IL-9 per lane. Primary antibody: Human IL-9 primary antibody at 1:1,000 overnight at 4oC. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 14 kDa for Human IL-9. Other band(s): None.
Product group Antibodies
GeneTex
IL9 antibody (Biotin)GTX48670
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL9
  • SizePrice
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