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Primary Antibodies

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Western blot using GeneTex's affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. Primary antibody was used at a 1:350 dilution followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG.
Product group Antibodies
GeneTex
NEDD4 antibodyGTX48706
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetNEDD4
  • SizePrice
Western blot using GeneTex's affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TBST overnight at 4oC. Primary antibody was used at a 1:1,000 dilution followed by reaction with a 1:10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection.
Product group Antibodies
GeneTex
Lac I antibodyGTX48707
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityBacteria
  • SizePrice
Western blot using GeneTex's affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.
Product group Antibodies
GeneTex
Mlf1 Interacting Protein antibodyGTX48708
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCENPU
  • SizePrice
Western blot using GeneTex's affinity purified anti-MLF1IP / PBIP1 antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.
Product group Antibodies
GeneTex
Mlf1 Interacting Protein antibodyGTX48709
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCENPU
  • SizePrice
Western blot using GeneTex Immuno-chemicals affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000.
Product group Antibodies
GeneTex
Pogz antibodyGTX48710
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetPogz
  • SizePrice
Western blot using GeneTex's affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.
Product group Antibodies
GeneTex
FAP antibodyGTX48711
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetFap
  • SizePrice
Western blot using GeneTex's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral shRNA knockdown vectors
Product group Antibodies
GeneTex
eIF3e antibodyGTX48712
ApplicationsWestern Blot, ELISA
ReactivityHuman, Monkey, Mouse
TargetEif3e
  • SizePrice
Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 μg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4oC Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa
Product group Antibodies
GeneTex
C/EBP delta antibodyGTX48713
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCebpd
  • SizePrice
Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
Product group Antibodies
GeneTex
IL7 Receptor alpha (phospho Tyr449) antibodyGTX48714
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIl7r
  • SizePrice
ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX48715 IL7 Receptor alpha antibody. Coating : 0.1 μg
Product group Antibodies
GeneTex
IL7 Receptor alpha antibodyGTX48715
ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetIl7r
  • SizePrice
Western blot using GeneTex's affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct western blot of wt splenocytes. Lane 6 shows direct western blot of knock out mouse.
Product group Antibodies
GeneTex
PSCDBP antibodyGTX48718
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetCytip
  • SizePrice
Western blot using GeneTex's affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
Product group Antibodies
GeneTex
ASPP1 antibodyGTX48719
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPPP1R13B
  • SizePrice
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