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Primary Antibodies

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Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetCcl20
  • SizePrice
Western blot using GeneTex's anti-Mouse MIP3a antibody shows detection of a band ~11 kDa in size corresponding to recombinant mouse MIP3a. Recombinant mouse MIP-3a was loaded on to an SDS-PAGE gel at 0.25 μg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4oC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reation with FemtoMax Chemiluminescent substrate. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetCcl20
  • SizePrice
Immunohistochemical staining of human cervical cancer tissue (40X magnification) using GeneTex's Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetJAG1
  • SizePrice
Ube2J2 antibody
Product group Antibodies
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetUBE2J2
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL17F
  • SizePrice
Western Blot of Peroxidase conjugated Rabbit anti-IL-17F Antibody (HRP). Load: 50 ng Human IL-17F per lane. Predicted/Observed size: 16kDa for Human IL-17F.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL17F
  • SizePrice
Western blot using GeneTex's anti-Human IL17F antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL17F (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetIL17F
  • SizePrice
GeneTex's Affinity Purified anti-iASPP antibody shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPPP1R13L
  • SizePrice
Western blot using GeneTex's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag? antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1,400.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human
TargetABCB6
  • SizePrice
Western blot demonstrating detection of NogoA in human brain tissue lysate. Primary antibody was used at concentration of 0.5 μg/mL (A) and 1.0 μg/mL (B).
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRTN4
  • SizePrice
Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20μg). Lane 2: NBLS Nuclear (3μg). Lane 3: BE2C Cytoplasmic (30μg). Lane 4: BE2C Nuclear (7μg). Lane 5: SY5Y-CASZ1b (10μg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman
TargetCASZ1
  • SizePrice
Western blot using GeneTex's affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45μm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, Other Application
ReactivityMonkey, Mouse
TargetMyb
  • SizePrice

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