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Proteins / Signaling Molecules

This product group contains both native proteins and recombinant proteins expressed and purified from bacterial, insect and mammalian expression systems. Bio-Connect also offers fusion proteins, peptides, enzymes, bacterial and viral proteins, hormones and growth factors. Furthermore, it includes chemokines and cytokines, collagenases, albumins, globulins, blood/plasma related proteins and more.

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Binding of GTX65631-pro IL2 (Superkine) human Fc fusion protein to IL-2Rbeta (human) is increased >10 fold compared to IL-2 human Fc fusion protein. Method: IL-2Rbeta (human) protein was coated on an ELISA plate at 1microg/ml. After blocking and washing steps, indicated concentrations of GTX65631-pro IL-2 Superkine (Fc) or IL-2 (Fc) protein were added. Following incubation for 1 h at RT, the binding was detected using an anti-human Fc antibody (HRP).
Product group Proteins / Signaling Molecules
GeneTex
Human IL2 (Superkine) + Human IgG2 Fc fusion proteinGTX65631-PRO
ApplicationsFunctional Assay
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GTX65633-pro CD137L protein (DDDDK tagged) binding to CD137 transfected cells. HEK 293 cells were transiently transfected with a construct encoding full-length mouse CD137. At 48hrs after transfection, the transfectants were incubated with 0.5 or 5microg/ml GTX65633 CD137L protein (DDDDK tagged) and followed by flow cytometry analysis with anti-DDDDK anibody.
Product group Proteins / Signaling Molecules
GeneTex
Mouse 4-1BBL / CD137L proteinGTX65633-PRO
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Pull down assay of GTX65634-pro CD137L protein (DDDDK tagged) by hCD137-Fc. 1. hCD137-Fc + hCD137L-DDDDDK-tag 2. hCD137-Fc + hGITRL-DDDDDK-tag 3. hCD137-Fc + hGITRL-His-tag 4. hGITR-Fc + hCD137L-DDDDDK-tag 5. hGITR-Fc + hGITRL-DDDDDK-tag
Product group Proteins / Signaling Molecules
GeneTex
Human 4-1BBL / CD137L proteinGTX65634-PRO
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GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100microl RPMI supplemented with 10% FCS) for 24 hours at 37oC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1microg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
Product group Proteins / Signaling Molecules
GeneTex
Human CD40L / CD154 protein (active, multimeric)GTX65635-PRO
ApplicationsFunctional Assay
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Adipogenesis inhibition of 3T3L-1 cells. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1microM, and 10microg/ml, respectively and continued for 2 days (day 0).The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5microg/ml of human Jagged1-Fc (GTX65636-pro) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Product group Proteins / Signaling Molecules
GeneTex
Human Jagged 1 + Human IgG1 Fc fusion proteinGTX65636-PRO
ApplicationsFunctional Assay
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Product group Proteins / Signaling Molecules
GeneTex
Human Calreticulin proteinGTX65637-PRO
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Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 microg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Product group Proteins / Signaling Molecules
GeneTex
Rat Progranulins proteinGTX65638-PRO
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Product group Proteins / Signaling Molecules
GeneTex
Human Netrin 1 + Human IgG1 Fc fusion proteinGTX65639-PRO
ApplicationsFunctional Assay
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Binding of LAG3 human Fc fusion protein (GTX65640-pro) on dendritic and B cells. Method: Monocyte-derived immature DC (iDC), DC matured for 48h with LPS (mDC) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10microg/ml) or GTX65640 LAG3 human Fc fusion protein (10microg/ml) for 30 min. at 4oC in PBS/1% BSA. The cells are then stained for 30 min. at 4oC with anti-human Fc antibody.
Product group Proteins / Signaling Molecules
GeneTex
Human LAG3 + Human IgG1 Fc fusion proteinGTX65640-PRO
ApplicationsFunctional Assay
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The effects on phospho-ERK1/2 and ERK1/2 by GTX65641-pro Progranulin protein in neuronal differentiated mouse P19 cells. Undifferentiated mouse P19 cells were induced to differentiated in 1microM retinoic acid (RA) in alpha-minimum essential medium (alphaMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3~4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated on tissue culture grade plates in the absence of RA for 3~4days. To examine the induction of signal of phospho-ERK1/2 and ERK1/2, reactions were carried out at 37oC over 0, 5, 10, 30, 60, 120mins, respectively by adding the recombinant protein (500ng/ml) to the neuronal differentiated mouse P19 cells, which were maintained with serum starvation for 24hrs. Treatment with Progranulin protein (GTX65641-pro) was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120mins, respectively. GAPDH was used as loading control for western blotting.
Product group Proteins / Signaling Molecules
GeneTex
Human Progranulins protein (active)GTX65641-PRO
ApplicationsFunctional Assay
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Deglycosylation of GTX65642-pro Progranulin (mouse) protein.To examine the deglycosylation of mouse Progranulin, 1 microg of mouse progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (mouse) antibody.
Product group Proteins / Signaling Molecules
GeneTex
Mouse Progranulins proteinGTX65642-PRO
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Product group Proteins / Signaling Molecules
GeneTex
Mouse Clusterin (Nuclear) proteinGTX65643-PRO
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