![FACS analysis of Daudi cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Daudi cells FACS analysis of Daudi cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Daudi cells](https://www.genetex.com/upload/website/prouct_img/normal/GTX80341/GTX80341_0521_FACS2_w_23061322_188.webp)
FACS analysis of Daudi cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Daudi cells
RPB1 antibody [4H8]
GTX80341
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityFish, Human
TargetPOLR2A
Overview
- SupplierGeneTex
- Product NameRPB1 antibody [4H8]
- Delivery Days Customer9
- Application Supplier NoteOptimal dilutions should be determined experimentally by the researcher.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4H8
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5430
- Target namePOLR2A
- Target descriptionRNA polymerase II subunit A
- Target synonymsNEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, DNA-directed RNA polymerase II subunit RPB1, 3'-5' exoribonuclease, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, polymerase (RNA) II subunit A
- HostMouse
- IsotypeIgG1
- Protein IDP24928
- Protein NameDNA-directed RNA polymerase II subunit RPB1
- Scientific DescriptionThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
- ReactivityFish, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Luteinizing hormone-induced expression of Ptger4b, a prostaglandin E2 receptor indispensable for ovulation of the medaka Oryzias latipes, is regulated by a genomic mechanism involving nuclear progestin receptor. Hagiwara A et al., 2014 Jun, Biol ReprodRead this paper
![WB analysis of various samples using GTX80341 RPB1 antibody [4H8]
Lane1: Hela
Lane2: MCF7
Lane3: NIH-3T3
Lane4: Cos7 WB analysis of various samples using GTX80341 RPB1 antibody [4H8]
Lane1: Hela
Lane2: MCF7
Lane3: NIH-3T3
Lane4: Cos7](https://www.genetex.com/upload/website/prouct_img/normal/GTX80341/GTX80341_0521_WB_w_23061322_669.webp)
![ICC/IF analysis of Hela cells using GTX80341 RPB1 antibody [4H8]
Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). ICC/IF analysis of Hela cells using GTX80341 RPB1 antibody [4H8]
Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](https://www.genetex.com/upload/website/prouct_img/normal/GTX80341/GTX80341_0521_ICCIF_w_23061322_747.webp)
![ICC/IF analysis of Hela cells using GTX80341 MCF7 antibody [4H8] ICC/IF analysis of Hela cells using GTX80341 MCF7 antibody [4H8]](https://www.genetex.com/upload/website/prouct_img/normal/GTX80341/GTX80341_0521_WB2_w_23061322_150.webp)
![FACS analysis of Hela cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Hela cells FACS analysis of Hela cells using GTX80341 RPB1 antibody [4H8]
Orange: Negative control
Blue: Hela cells](https://www.genetex.com/upload/website/prouct_img/normal/GTX80341/GTX80341_0521_FACS_w_23061322_222.webp)






![WB analysis of MCF7 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03214 RPB1 CTD (phospho Ser2) antibody [GT1302]. Dilution : 1:1000 Loading : 25μg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03214/GTX03214_69_WB_w_23053123_250.webp)
![WB analysis of MCF7 cells were treated by Doxorubicin (0.5 uM) at 37oC for 24 hours or treated by nocodazole (50 ng/mL) at 37oC for 20 hours or treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303]. Dilution : 1:1000 Loading : 25μg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03215/GTX03215_73_WB_w_23053123_365.webp)