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ProductsBack/RPB1 CTD (phospho Ser5) antibody [GT1303]
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RPB1 CTD (phospho Ser5) antibody [GT1303]

GTX03215
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPOLR2A
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPB1 CTD (phospho Ser5) antibody [GT1303] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1303
  • Concentration
    0.25 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5430
  • Target name
    POLR2A
  • Target description
    RNA polymerase II subunit A
  • Target synonyms
    DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24928
  • Protein Name
    DNA-directed RNA polymerase II subunit RPB1
  • Scientific Description
    This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2A using anti-POLR2A antibody (A01029-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A antigen affinity purified polyclonal antibody (Catalog # A01029-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2A at approximately 220KD. The expected band size for POLR2A is at 220KD.
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