Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of MCF7 cells were treated by Doxorubicin (0.5 uM) at 37oC for 24 hours or treated by nocodazole (50 ng/mL) at 37oC for 20 hours or treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03215/GTX03215_73_WB_w_23053123_365.webp "WB analysis of MCF7 cells were treated by Doxorubicin (0.5 uM) at 37oC for 24 hours or treated by nocodazole (50 ng/mL) at 37oC for 20 hours or treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303]. Dilution : 1:1000 Loading : 25microg per lane")
![WB analysis of C2C12 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03215/GTX03215_74_WB_w_23053123_355.webp "WB analysis of C2C12 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RPB1 CTD (phospho Ser5) antibody [GT1303]
GTX03215
Overview
- SupplierGeneTex
- Product NameRPB1 CTD (phospho Ser5) antibody [GT1303] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1303
- Concentration0.25 mg/ml
- ConjugateUnconjugated
- Gene ID5430
- Target namePOLR2A
- Target descriptionRNA polymerase II subunit A
- Target synonymsDNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
- HostRabbit
- IsotypeIgG
- Protein IDP24928
- Protein NameDNA-directed RNA polymerase II subunit RPB1
- Scientific DescriptionThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203