![Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser2) antibody [GT1302] (GTX03214) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser2) antibody [GT1302] (GTX03214) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03214/GTX03214_4000001540_20210716_WB_CIP_w_23053123_320.webp)
Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser2) antibody [GT1302] (GTX03214) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RPB1 CTD (phospho Ser2) antibody [GT1302]
GTX03214
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOLR2A
Overview
- SupplierGeneTex
- Product NameRPB1 CTD (phospho Ser2) antibody [GT1302] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1302
- Concentration0.75 mg/ml
- ConjugateUnconjugated
- Gene ID5430
- Target namePOLR2A
- Target descriptionRNA polymerase II subunit A
- Target synonymsDNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
- HostRabbit
- IsotypeIgG
- Protein IDP24928
- Protein NameDNA-directed RNA polymerase II subunit RPB1
- Scientific DescriptionThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203