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Search results: ChIP

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Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetHMGA2
  • SizePrice
ChIP analysis of sheared chromatin from 1.25x10? SKNO-1 cells using GTX60333 CBFb antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. This figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used. Antibody amount : 4microl
Product group Antibodies
GeneTex
ApplicationsChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetCBFB
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ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
References
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetMECP2
  • SizePrice
ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60359 MBD1 antibody - ChIP grade. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Mouse
TargetMBD1
  • SizePrice
WB analysis of nuclear extracts (20 microg) from HeLa cells using GTX60362 LSD1 antibody - ChIP grade. Dilution : 1:1,000
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
ReactivityHuman
TargetKDM1A
  • SizePrice
ChIP was performed with mouse ESCs nuclear extract and either 10 microg of SUZ12 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Sox1 promoter or Actin.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
ReactivityHuman, Mouse
TargetSUZ12
  • SizePrice
ChIP analysis of sheared chromatin from 2x10? NALM cells using GTX60368 RFXAP antibody - ChIP grade. An anti-flag antibody (lane 2) was used as negative IP control. This figure shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). Antibody amount : 5-7microg
Product group Antibodies
GeneTex
ApplicationsChIP Chromatin ImmunoPrecipitation
ReactivityHuman
TargetRFXAP
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WB analysis of whole cell extracts (WCE) from drosophila larva and drosophila adults (Figure 1A) or on nuclear extracts from HeLa cells (HeLa NE, 20 microg) (Figure 1B) using GTX60804 CDC73 antibody - ChIP grade. Dilution : 1:1,000
Product group Antibodies
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation
ReactivityDrosophila, Human
TargetCDC73
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ChIP analysis of sheared chromatin from 4x10? K562 cells using GTX60811 EZH2 antibody - ChIP grade. A titration of the antibody consisting of 1, 2. 5 and 10 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, Other Application
ReactivityHuman, Mouse
TargetEzh2
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WB analysis of whole cell extracts (WCE) from drosophila larva and drosophila adults using GTX60844 Rtf1 antibody - ChIP grade. Dilution : 1:1000
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
ReactivityDrosophila
TargetRTF1
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WB analysis of nuclear extract from HeLa cells (lane 1), Drosophila adult whole cell extracts (lane 2), and the purified human PAF complex (lane 3) (Figure 1A) or on nuclear extracts from HeLa cells (HeLa NE, 20 microg) (Figure 1B) using GTX60846 WDR61 antibody - ChIP grade. Dilution : 1:1,000
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
ReactivityDrosophila, Human
TargetWDR61
  • SizePrice
ChIP analysis of HEK293T and NIH3T3 cells using GTX60851 TIP-5 antibody - ChIP grade. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4?C with 100 microg sheared chromatin and either 5 microl of the TIP5 antibody or 5 microl IgG which was used as negative IP control. The IPd DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. This figure shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
ReactivityHuman, Mouse
TargetBAZ2A
  • SizePrice