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ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX32260 SEK1 / MKK4 (phospho Thr261) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX32260 SEK1 / MKK4 (phospho Thr261) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX32260 SEK1 / MKK4 (phospho Thr261) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

SEK1 / MKK4 (phospho Thr261) antibody

GTX32260
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAP2K4
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEK1 / MKK4 (phospho Thr261) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6416
  • Target name
    MAP2K4
  • Target description
    mitogen-activated protein kinase kinase 4
  • Target synonyms
    c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; MKK4; PRKMK4; SAPK/ERK kinase 1; SAPKK1; SAPKK-1; SEK1; SERK1; SKK1; stress-activated protein kinase kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45985
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 4
  • Scientific Description
    This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
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