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ProductsBack/SOD1 (mutant) antibody [MS785]
ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1microg/ml (12 hours at 4oC)
ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1microg/ml (12 hours at 4oC)
ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1microg/ml (12 hours at 4oC)

SOD1 (mutant) antibody [MS785]

GTX57211
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSOD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOD1 (mutant) antibody [MS785]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1 microg/ml (1/500 dilution) -5 microg/ml (1/100 dilution). IP: 1-5microg/sample. IHC: 1 microg/ml (1/500 dilution) -5 microg/ml (1/100 dilution). *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MS785
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6647
  • Target name
    SOD1
  • Target description
    superoxide dismutase 1
  • Target synonyms
    ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer, superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SOD, soluble, epididymis secretory protein Li 44, indophenoloxidase A, superoxide dismutase 1, soluble, superoxide dismutase, cystolic
  • Host
    Rat
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kurlawala Z, McMillan JD, Singhal RA, et al. Mutant and curli-producing E. coli enhance the disease phenotype in a hSOD1-G93A mouse model of ALS. Sci Rep. 2023,13(1):5945. doi: 10.1038/s41598-023-32594-5
    Read this paper
  • Fujisawa T, Yamaguchi N, Kadowaki H, et al. A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants. Neurobiol Dis. 2015,82:478-486. doi: 10.1016/j.nbd.2015.08.010
    Read this paper
  • Fujisawa T, Homma K, Yamaguchi N, et al. A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants. Ann Neurol. 2012,72(5):739-49. doi: 10.1002/ana.23668
    Read this paper

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Figure 1. Western blot analysis of SOD1 using anti-SOD1 antibody (PB9402). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PB9402) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.
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