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ProductsBack/SOD1 antibody [AT6F1]
FACS analysis of Hep3B cells using GTX57658 SOD1 antibody. Cell Number: 1 x 10? cells Primary antibody: Red line Antibody amount: 2-5 microg
FACS analysis of Hep3B cells using GTX57658 SOD1 antibody. Cell Number: 1 x 10? cells Primary antibody: Red line Antibody amount: 2-5 microg
FACS analysis of Hep3B cells using GTX57658 SOD1 antibody. Cell Number: 1 x 10? cells Primary antibody: Red line Antibody amount: 2-5 microg

SOD1 antibody [AT6F1]

GTX57658
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSOD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOD1 antibody [AT6F1]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT6F1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6647
  • Target name
    SOD1
  • Target description
    superoxide dismutase 1
  • Target synonyms
    ALS; ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; homodimer; hSod1; indophenoloxidase A; IPOA; SOD; SOD, soluble; STAHP; superoxide dismutase [Cu-Zn]; superoxide dismutase 1, soluble; superoxide dismutase, cystolic
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP00441
  • Protein Name
    Superoxide dismutase [Cu-Zn]
  • Scientific Description
    The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1alpha Inhibition. Osuru HP et al., 2022, Front Cardiovasc Med
    Read more

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Figure 1. Western blot analysis of SOD1 using anti-SOD1 antibody (PB9402). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PB9402) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.
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