More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SPHK1 Rabbit pAb

SPHK1 Rabbit pAb

A0139
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSPHK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    ABclonal
  • Product Name
    SPHK1 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8877
  • Target name
    SPHK1
  • Target description
    sphingosine kinase 1
  • Target synonyms
    SPHK, sphingosine kinase 1, SK 1, SPK 1, acetyltransferase SPHK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYA1
  • Protein Name
    Sphingosine kinase 1
  • Reactivity
    Human, Mouse
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
SPHK1 AntibodyCAB0139
ReactivityHuman
TargetSPHK1
  • SizePrice
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SPHK1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 52, 44 kDa Observed band size: 43 kDa
Product group Antibodies
Cusabio
SPHK1 AntibodyCSB-PA022564LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSPHK1
  • SizePrice
Figure 1. Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 45-50 kDa.
Product group Antibodies
Boster Bio
Anti-SPHK1 Antibody Picoband(r)A01390-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPHK1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SPHK1 Antibody144-00139
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPHK1
  • SizePrice
Anti-SPHK1 Antibody
Product group Antibodies
Antibodies.com
Anti-SPHK1 AntibodyA83416
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SPHK / SPHK1 AntibodyLS-C746745
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetSPHK1
  • SizePrice
Product group Antibodies
MedChem Express
SPHK1 Antibody (YA063, Rabbit)HY-P80337
TargetSPHK1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-SPHK1EB08409
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Rat
TargetSPHK1
  • SizePrice
Immunofluorescence analysis of paraformaldehyde-fixed A431, using SPHK1(GTX104724) antibody at 1:200 dilution.
Product group Antibodies
GeneTex
SPHK1 antibody [C3], C-termGTX104724
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetSPHK1
  • SizePrice
Product group Antibodies
Biosynth
SPHK1 antibody20R-SR024
  • SizePrice