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ProductsBack/TGFalpha(1E8-G6), 1mg/mL [26628-22-8]
TGFalpha(1E8-G6), 1mg/mL [26628-22-8]

TGFalpha(1E8-G6), 1mg/mL [26628-22-8]

BNUM0785
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Zebra Fish
TargetTGFA
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Overview

  • Supplier
    Biotium
  • Product Name
    TGFalpha(1E8-G6), 1mg/mL [26628-22-8]
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • CAS Number
    26628-22-8
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1E8-G6
  • Concentration
    1 mg/ml
  • Gene ID7039
  • Target name
    TGFA
  • Target description
    transforming growth factor alpha
  • Target synonyms
    TFGA, protransforming growth factor alpha, TGF-alpha
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDP01135
  • Protein Name
    Protransforming growth factor alpha
  • Scientific Description
    This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.
  • Source
    Animal
  • Reactivity
    Human, Mouse, Rabbit, Zebra Fish
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of TGFA using anti-TGFA antibody (M01779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFA antigen affinity purified monoclonal antibody (Catalog # M01779-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFA at approximately 17 kDa. The expected band size for TGFA is at 17 kDa.
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