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ProductsBack/TNF alpha antibody [F6C5]
Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [F6C5] (GTX28349) (1 μg/mL). Please notice that GTX28349 needs to be conjugated to biotin to function as the detection antibody. Please contact us for custom biotin-conjugated antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [F6C5] (GTX28349) (1 μg/mL). Please notice that GTX28349 needs to be conjugated to biotin to function as the detection antibody. Please contact us for custom biotin-conjugated antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [F6C5] (GTX28349) (1 μg/mL). Please notice that GTX28349 needs to be conjugated to biotin to function as the detection antibody. Please contact us for custom biotin-conjugated antibody.

TNF alpha antibody [F6C5]

GTX28349
GeneTex
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNF
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Overview

  • Supplier
    GeneTex
  • Product Name
    TNF alpha antibody [F6C5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F6C5
  • Conjugate
    Unconjugated
  • Gene ID7124
  • Target name
    TNF
  • Target description
    tumor necrosis factor
  • Target synonyms
    DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, tumor necrotic factor alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01375
  • Protein Name
    Tumor necrosis factor
  • Scientific Description
    This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

References

  • Astrocyte-specific activation of TNFR2 promotes oligodendrocyte maturation by secretion of leukemia inhibitory factor. Fischer R et al., 2014 Feb, Glia
    Read this paper

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
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