Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [F6C5] (GTX28349) (1 microg/mL). Please notice that GTX28349 needs to be conjugated to biotin to function as the detection antibody. Please contact us for custom biotin-conjugated antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [F6C5] (GTX28349) (1 microg/mL). Please notice that GTX28349 needs to be conjugated to biotin to function as the detection antibody. Please contact us for custom biotin-conjugated antibody.
TNF alpha antibody [F6C5]
GTX28349
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNF
Overview
- SupplierGeneTex
- Product NameTNF alpha antibody [F6C5]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDF6C5
- ConjugateUnconjugated
- Gene ID7124
- Target nameTNF
- Target descriptiontumor necrosis factor
- Target synonymsDIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, tumor necrotic factor alpha
- HostMouse
- IsotypeIgG1
- Protein IDP01375
- Protein NameTumor necrosis factor
- Scientific DescriptionThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Fischer R, Wajant H, Kontermann R, et al. Astrocyte-specific activation of TNFR2 promotes oligodendrocyte maturation by secretion of leukemia inhibitory factor. Glia. 2014,62(2):272-83. doi: 10.1002/glia.22605Read this paper
Datasheet
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