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TNFR1/TNFRSF1A Rabbit pAb

A1540
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNFRSF1A
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Overview

  • Supplier
    ABclonal
  • Product Name
    TNFR1/TNFRSF1A Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7132
  • Target name
    TNFRSF1A
  • Target description
    TNF receptor superfamily member 1A
  • Target synonyms
    CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60, tumor necrosis factor receptor superfamily member 1A, TNF-R1, TNF-RI, TNFR-I, tumor necrosis factor binding protein 1, tumor necrosis factor receptor type 1, tumor necrosis factor-alpha receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19438
  • Protein Name
    Tumor necrosis factor receptor superfamily member 1A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
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