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ProductsBack/WNT10B Mouse Monoclonal Antibody [Clone ID: 5A7]
Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1).
Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1).
Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1).

WNT10B Mouse Monoclonal Antibody [Clone ID: 5A7]

AM06372SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWNT10B
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    WNT10B Mouse Monoclonal Antibody [Clone ID: 5A7]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A7
  • Gene ID7480
  • Target name
    WNT10B
  • Target description
    Wnt family member 10B
  • Target synonyms
    SHFM6, STHAG8, WNT-12, protein Wnt-10b, WNT-10B protein, wingless-type MMTV integration site family, member 10B
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO00744
  • Protein Name
    Protein Wnt-10b
  • Scientific Description
    WNT10B mouse monoclonal antibody, clone 5A7, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.
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