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ProductsBack/ZO-1 antibody [N1N2], N-term

ZO-1 antibody [N1N2], N-term

GTX108613
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityFish, Human, Mouse, Rat
TargetTJP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZO-1 antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.58 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7082
  • Target name
    TJP1
  • Target description
    tight junction protein 1
  • Target synonyms
    ZO-1, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07157
  • Protein Name
    Tight junction protein 1
  • Scientific Description
    This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
  • Reactivity
    Fish, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hsia Y, Sivasubramanian M, Chu CH, et al. A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis. Int J Nanomedicine. 2023,18:4253-4274. doi: 10.2147/IJN.S412932
    Read this paper
  • Su WP, Li CJ, Lin LT, et al. Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition. Biomed Pharmacother. 2023,163:114888. doi: 10.1016/j.biopha.2023.114888
    Read this paper
  • Möckel M, Baldok N, Walles T, et al. Human 3D Airway Tissue Models for Real-Time Microscopy: Visualizing Respiratory Virus Spreading. Cells. 2022,11(22). doi: 10.3390/cells11223634
    Read this paper
  • Russell DF, Zhang W, Warnock TC, et al. Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon. PLoS One. 2022,17(11):e0276854. doi: 10.1371/journal.pone.0276854
    Read this paper
  • Odom MR, Hughes FM Jr, Jin H, et al. Diabetes causes NLRP3-dependent barrier dysfunction in mice with detrusor overactivity but not underactivity. Am J Physiol Renal Physiol. 2022,323(6):F616-F632. doi: 10.1152/ajprenal.00047.2022
    Read this paper
  • Zhang MH, Liu J. Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway. Bioengineered. 2022,13(4):10047-10060. doi: 10.1080/21655979.2022.2063100
    Read this paper
  • Liu X, Chen B, Chen J, et al. Deubiquitinase ubiquitin-specific peptidase 10 maintains cysteine rich angiogenic inducer 61 expression via Yes1 associated transcriptional regulator to augment immune escape and metastasis of pancreatic adenocarcinoma. Cancer Sci. 2022,113(5):1868-1879. doi: 10.1111/cas.15326
    Read this paper
  • Yen YT, Yang JC, Chang JB, et al. Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells. Int J Mol Sci. 2021,23(1). doi: 10.3390/ijms23010325
    Read this paper
  • Russell DF, Warnock TC, Zhang W, et al. Large-Scale Convergence of Receptor Cell Arrays Onto Afferent Terminal Arbors in the Lorenzinian Electroreceptors of Polyodon. Front Neuroanat. 2020,14:50. doi: 10.3389/fnana.2020.00050
    Read this paper
  • Voirin AC, Celle S, Perek N, et al. Sera of elderly obstructive sleep apnea patients alter blood-brain barrier integrity in vitro: a pilot study. Sci Rep. 2020,10(1):11309. doi: 10.1038/s41598-020-68374-8
    Read this paper

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Figure 1. Western blot analysis of TJP1 using anti-TJP1 antibody (PB9234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody (Catalog # PB9234) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 185 kDa.
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