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ProductsBack/Mouse anti Human vitronectin receptor CD51

Mouse anti Human vitronectin receptor CD51

MUB2011P
Nordic-MUbio
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetITGAV
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human vitronectin receptor CD51
  • Delivery Days Customer
    7
  • Application Supplier Note
    NKI-M7 is useful for ELISA, immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    ELISA;Flow Cytometry;Immunohistochemistry (frozen);Immunoprecipitation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NKI-M7 (former AMF7)
  • Gene ID3685
  • Target name
    ITGAV
  • Target description
    integrin subunit alpha V
  • Target synonyms
    CD51, MSK8, VNRA, VTNR, integrin alpha-V, antigen identified by monoclonal antibody L230, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), vitronectin receptor subunit alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06756
  • Protein Name
    Integrin alpha-V
  • Source
    NKI-M7 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin alpha V/ITGAV using anti-Integrin alpha V/ITGAV antibody (A01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha V/ITGAV antigen affinity purified polyclonal antibody (Catalog # A01561-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha V/ITGAV at approximately 130-140KD. The expected band size for Integrin alpha V/ITGAV is at 130KD.
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