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WB analysis of E. coli expressing a multi-tag fusion protein using GTX12213 c-Myc antibody (HRP). Dilution : 0.04μg/ml
WB analysis of E. coli expressing a multi-tag fusion protein using GTX12213 c-Myc antibody (HRP). Dilution : 0.04μg/ml
WB analysis of E. coli expressing a multi-tag fusion protein using GTX12213 c-Myc antibody (HRP). Dilution : 0.04μg/ml

c-Myc antibody (HRP)

GTX12213
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYC
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Overview

  • Supplier
    GeneTex
  • Product Name
    c-Myc antibody (HRP)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1,000 - 1:30,000. ICC/IF: 1:200-1:500. ELISA: 1:10,000-1:100,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Gene ID4609
  • Target name
    MYC
  • Target description
    MYC proto-oncogene, bHLH transcription factor
  • Target synonyms
    MRTL, MYCC, bHLHe39, c-Myc, myc proto-oncogene protein, avian myelocytomatosis viral oncogene homolog, class E basic helix-loop-helix protein 39, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP01106
  • Protein Name
    Myc proto-oncogene protein
  • Scientific Description
    This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C-Myc/MYC using anti-C-Myc/MYC antibody (A00026-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Myc/MYC antigen affinity purified polyclonal antibody (Catalog # A00026-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Myc/MYC at approximately 65 kDa. The expected band size for C-Myc/MYC is at 65 kDa.
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