![Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03207/GTX03207_4000001533_20210716_WB_M_CIP_w_23053123_302.webp)
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
c-Myc (phospho Ser62) antibody [GT1295]
GTX03207
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMYC
Overview
- SupplierGeneTex
- Product Namec-Myc (phospho Ser62) antibody [GT1295]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1295
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4609
- Target nameMYC
- Target descriptionMYC proto-oncogene, bHLH transcription factor
- Target synonymsMRTL, MYCC, bHLHe39, c-Myc, myc proto-oncogene protein, avian myelocytomatosis viral oncogene homolog, class E basic helix-loop-helix protein 39, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog
- HostRabbit
- IsotypeIgG
- Protein IDP01106
- Protein NameMyc proto-oncogene protein
- Scientific DescriptionThis gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Ser62) antibody [GT1295] (GTX03207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03207/GTX03207_4000001533_20210716_WB_CIP_w_23053123_272.webp)
![WB analysis of 293T cells were treated by Forskolin (10 uM) at 37oC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295]. Dilution : 1:1000 Loading : 25μg per lane WB analysis of 293T cells were treated by Forskolin (10 uM) at 37oC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295]. Dilution : 1:1000 Loading : 25μg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03207/GTX03207_67_WB_w_23053123_105.webp)
![WB analysis of NIH-3T3 cells were treated by Forskolin (30 uM) at 37oC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295]. Dilution : 1:1000 Loading : 25μg per lane WB analysis of NIH-3T3 cells were treated by Forskolin (30 uM) at 37oC for 30 minutes after serum-starvation overnight using GTX03207 c-Myc (phospho Ser62) antibody [GT1295]. Dilution : 1:1000 Loading : 25μg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03207/GTX03207_68_WB_w_23053123_612.webp)



![ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10]. Dilution : 1:2000 in 1% BSA in PBS at 4 degree C, overnight Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX00684/GTX00684_20191104_ICC-IF_w_23053121_372.webp)
![Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc (phospho Thr58/Ser62) antibody [SZ02-06] (GTX01137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01137/GTX01137_HK1219_20200228_WB_treatment_MG132_w_23053121_696.webp)
![WB analysis of mouse ovary tissue lysate using GTX02877 c-Myc antibody [GT1265]. Dilution : 1:1000 Loading : 25μg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX02877/GTX02877_20210226_WB_2_w_23053123_529.webp)
![IHC-P analysis of human colon adenocarcinoma tissue using GTX04712 c-Myc antibody [9E10]. Antigen retrieval : Citrate Buffer pH 6.0_x000D_](https://www.genetex.com/upload/website/prouct_img/normal/GTX04712/GTX04712_20240325_IHC-P_24032422_493.webp)

![FACS (intracellular staining) analysis of transfected LST-1-c-Myc in HEK-293 cells compared with nontransfected HEK-293 cells using GTX10826 c-Myc antibody [9E10] (FITC).](https://www.genetex.com/upload/website/prouct_img/normal/GTX10826/GTX10826_20191028_FACS_1_w_23060120_474.webp)