ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10] (Green, Left) or proximity Ligation Analysis with anti-c-Myc pS62 and CIP2A antibodies, association of c-Myc pS62 with CIP2A (Right, red) in nuclei (DAPI, blue).
![ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10]. Dilution : 1:2000 in 1% BSA in PBS at 4 degree C, overnight Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX00684/GTX00684_20191104_ICC-IF_w_23053121_372.webp "ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10]. Dilution : 1:2000 in 1% BSA in PBS at 4 degree C, overnight Fixation : 4% PFA, overnight Permeabilization : 0.25% Triton X-100 in PBS for 10 min")
![WB analysis of various samples using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10]. Scrambled siRNA : AGS cells transfected with scrambled siRNA Negative control siRNA : AGS cells transfected with negative control siRNA Myc1 : AGS cells transfected with c-Myc siRNA](https://www.genetex.com/upload/website/prouct_img/normal/GTX00684/GTX00684_20191104_WB_w_23053121_749.webp "WB analysis of various samples using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10]. Scrambled siRNA : AGS cells transfected with scrambled siRNA Negative control siRNA : AGS cells transfected with negative control siRNA Myc1 : AGS cells transfected with c-Myc siRNA")
ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10] (Green, Left) or proximity Ligation Analysis with anti-c-Myc pS62 and CIP2A antibodies, association of c-Myc pS62 with CIP2A (Right, red) in nuclei (DAPI, blue).
c-Myc (phospho Ser62) antibody [33A12E10] - KO/KD Validated
GTX00684
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMYC
Overview
- SupplierGeneTex
- Product Namec-Myc (phospho Ser62) antibody [33A12E10] - KO/KD Validated
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID33A12E10
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4609
- Target nameMYC
- Target descriptionMYC proto-oncogene, bHLH transcription factor
- Target synonymsavian myelocytomatosis viral oncogene homolog; bHLHe39; class E basic helix-loop-helix protein 39; c-Myc; MRTL; myc proto-oncogene protein; MYCC; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
- HostMouse
- IsotypeIgG2b
- Modification TypePhosphorylated
- Scientific DescriptionThis gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
- ReactivityHuman, Mouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- A regulatory loop among CD276, miR-29c-3p, and Myc exists in cancer cells against natural killer cell cytotoxicity. Lee CC et al., 2021 Jul 15, Life SciRead more
- Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation. Myant K et al., 2015 Aug 11, Cell RepRead more
- Studying c-Myc serine 62 phosphorylation in leukemia cells: concern over antibody cross-reactivity. Tibbitts DC et al., 2012 May 31, BloodRead more
- Identification of a c-Jun N-terminal kinase-2-dependent signal amplification cascade that regulates c-Myc levels in ras transformation. Mathiasen DP et al., 2012 Jan 19, OncogeneRead more
- Provisional restorations. Part Two. Tallents RH et al., 1988 Feb, Oral HealthRead more