ProductsBack/c-Myc (phospho Ser62) antibody [33A12E10]

ICC/IF analysis of HeLa cells using GTX00684 c-Myc (phospho Ser62) antibody [33A12E10] (Green, Left) or proximity Ligation Analysis with anti-c-Myc pS62 and CIP2A antibodies, association of c-Myc pS62 with CIP2A (Right, red) in nuclei (DAPI, blue).

c-Myc (phospho Ser62) antibody [33A12E10]

GTX00684

ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHuman, Mouse

TargetMYC

Overview

Supplier
GeneTex
Product Name
c-Myc (phospho Ser62) antibody [33A12E10]
Delivery Days Customer
9
Application Supplier Note
WB: ~1 microg/ml. ICC/IF: 0.5-1 microg/ml. IHC-P: 5 microg/ml. FACS: using 1 microg for 106 cells.. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
33A12E10
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID4609
Target name
MYC
Target description
MYC proto-oncogene, bHLH transcription factor
Target synonyms
MRTL, MYCC, bHLHe39, c-Myc, myc proto-oncogene protein, avian myelocytomatosis viral oncogene homolog, class E basic helix-loop-helix protein 39, myc-related translation/localization regulatory factor, proto-oncogene c-Myc, transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog
Host
Mouse
Isotype
IgG2b
Protein IDP01106
Protein Name
Myc proto-oncogene protein
Scientific Description
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Reactivity
Human, Mouse
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Lee CC, Ho KH, Huang TW, et al. A regulatory loop among CD276, miR-29c-3p, and Myc exists in cancer cells against natural killer cell cytotoxicity. Life Sci. 2021,277:119438. doi: 10.1016/j.lfs.2021.119438
Read this paper
Myant K, Qiao X, Halonen T, et al. Serine 62-Phosphorylated MYC Associates with Nuclear Lamins and Its Regulation by CIP2A Is Essential for Regenerative Proliferation. Cell Rep. 2015,12(6):1019-31. doi: 10.1016/j.celrep.2015.07.003
Read this paper
Tibbitts DC, Escamilla-Powers JR, Zhang X, et al. Studying c-Myc serine 62 phosphorylation in leukemia cells: concern over antibody cross-reactivity. Blood. 2012,119(22):5334-5. doi: 10.1182/blood-2012-03-414532
Read this paper
Mathiasen DP, Egebjerg C, Andersen SH, et al. Identification of a c-Jun N-terminal kinase-2-dependent signal amplification cascade that regulates c-Myc levels in ras transformation. Oncogene. 2012,31(3):390-401. doi: 10.1038/onc.2011.230
Read this paper
Tallents RH, Jarvis R. Provisional restorations. Part Two. Oral Health. 1988,78(2):29-33.
Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

c-Myc (phospho Ser62) antibody [33A12E10]

Large volume orders?

Overview

References

Datasheet

FAQ

Related products