
Hsp70 antibody detects Hsp70 protein on whole mount zebrafish by immunohistochemical analysis. Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo. Green: Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70oC
Hsp70 antibody
GTX112963
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.84 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsHEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, heat shock 70 kDa protein 1A, HSP70-1/HSP70-2, HSP70.1/HSP70.2, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, dnaK-type molecular chaperone HSP70-1, epididymis secretory protein Li 103, epididymis secretory sperm binding protein, heat shock 70 kDa protein 1, heat shock 70 kDa protein 1/2, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1A, heat shock 70kDa protein 1A, heat shock protein family A member 1A, heat shock-induced protein
- HostRabbit
- IsotypeIgG
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- Scientific DescriptionThis intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Caruso G, Scalisi EM, Pecoraro R, et al. Effects of carnosine on the embryonic development and TiO(2) nanoparticles-induced oxidative stress on Zebrafish. Front Vet Sci. 2023,10:1148766. doi: 10.3389/fvets.2023.1148766Read this paper

![Hsp70 antibody detects Hsp70 protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Hsp70 antibody detects Hsp70 protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX112963/GTX112963_43565_20220311_ICC_IF_w_23060500_420.webp)









![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody [HL1580] (GTX637059) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637059/GTX637059_T-44718_20220708_WB_M_R_22071401_933.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody [HL1581] (GTX637060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637060/GTX637060_44767_20220812_WB_22081423_752.webp)