![ICC/IF analysis of HeLa cells using GTX14210 PLK1 antibody [Mixed clones]. Dilution : 1microg/ml ICC/IF analysis of HeLa cells using GTX14210 PLK1 antibody [Mixed clones]. Dilution : 1microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_ICC-IF_3_w_23060620_538.webp)
ICC/IF analysis of HeLa cells using GTX14210 PLK1 antibody [Mixed clones]. Dilution : 1microg/ml
PLK1 antibody [Mixed clones]
GTX14210
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPLK1
Overview
- SupplierGeneTex
- Product NamePLK1 antibody [Mixed clones]
- Delivery Days Customer9
- Application Supplier NoteWB: 1-2microg/ml. ICC/IF: 1-5microg/ml. IHC-P: 1:50-1:100. FACS: 3-5microg/ 106 cells. IP: 5microg. ELISA: 0.1-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Clone IDMixed clones
- ConjugateUnconjugated
- Gene ID5347
- Target namePLK1
- Target descriptionpolo like kinase 1
- Target synonymsPLK, STPK13, serine/threonine-protein kinase PLK1, PLK-1, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, serine/threonine-protein kinase 13
- HostMouse
- IsotypeIgG1, IgG2b
- Protein IDP53350
- Protein NameSerine/threonine-protein kinase PLK1
- Scientific DescriptionThe Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human colon tissue using GTX14210 PLK1 antibody [Mixed clones]. Right : Primary antibody Left : Negative control without primary antibody Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20 IHC-P analysis of human colon tissue using GTX14210 PLK1 antibody [Mixed clones]. Right : Primary antibody Left : Negative control without primary antibody Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_IHC-P_20_w_23060620_745.webp)
![IHC-P analysis of mouse colon tissue using GTX14210 PLK1 antibody [Mixed clones]. Right : Primary antibody Left : Negative control without primary antibody Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:50 IHC-P analysis of mouse colon tissue using GTX14210 PLK1 antibody [Mixed clones]. Right : Primary antibody Left : Negative control without primary antibody Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_IHC-P_21_w_23060620_531.webp)
![WB analysis of various samples using GTX14210 PLK1 antibody [Mixed clones]. Lane1 : HEK-293 Lane2 : K562 Lane3 : A549 Lane4 : HT-29 Lane5 : COLO 205 Lane6 : PC-12 Lane7 : Cos-7 Lane8 : NIH3T3 Lane9 : A-431 Lane10 : HeLa Loading : 20microg Dilution : 1-2microg/ml WB analysis of various samples using GTX14210 PLK1 antibody [Mixed clones]. Lane1 : HEK-293 Lane2 : K562 Lane3 : A549 Lane4 : HT-29 Lane5 : COLO 205 Lane6 : PC-12 Lane7 : Cos-7 Lane8 : NIH3T3 Lane9 : A-431 Lane10 : HeLa Loading : 20microg Dilution : 1-2microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX14210/GTX14210_20191203_WB_41_w_23060620_283.webp)
![IHC-P analysis of human colon tissue using GTX15779 PLK1 antibody [13E8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX15779/GTX15779_1039_IHC-P_w_23060620_913.webp)
![WB analysis of HeLa treated with nocodazole lysate using GTX17683 PLK1 (phospho Thr210) antibody [HAO-16]. Lane 1 : HeLa Lane 2 : HeLa treated with nocodazole Loading : 50microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX17683/GTX17683_20190522_WB_w_23060620_598.webp)

![ELISA analysis of antigen using GTX60624 PLK1 antibody [1D1]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60624/GTX60624_20170912_ELISA_w_23061123_595.webp)
![ELISA analysis of antigen using GTX60627 PLK1 antibody [3C11]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60627/GTX60627_20170912_ELISA_w_23061123_775.webp)
![IHC-P analysis of squamous cell carcinoma of oropharangeal tissue using GTX01927 PLK1 antibody [MJS1]. Note the intense nuclear and cytoplasmic staining of a proportion of proliferating malignant cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01927/GTX01927_20200811_IHC-P_73_w_23053121_314.webp)
![FACS analysis of HEK293T cells transfected with either PLK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83864 PLK1 antibody [3F12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83864/GTX83864_204_FACS_w_23061420_168.webp)
![IHC-P analysis of human breast adenocarcinoma tissue using GTX83865 PLK1 antibody [1D4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83865/GTX83865_1943_IHC-P_w_23061420_628.webp)
![ICC/IF analysis of COS7 cells transiently transfected with PLK1 plasmid using GTX83866 PLK1 antibody [8C12]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX83866/GTX83866_1230_ICCIF_w_23061420_550.webp)