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Primary Antibodies

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Western blot showing detection of 0.1 μg of recombinant p39 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p39 fusion protein (expected MW: 77.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
Borrelia burgdorferi p39 antibodyGTX48801
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetBB_RS01885
  • SizePrice
Western blot showing detection of 0.1 μg of recombinant OspB protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS ) overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
OspB antibodyGTX48803
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetospB
  • SizePrice
Western blot showing detection of 0.1 μg of recombinant OspA protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
Borrelia burgdorferi Osp A antibodyGTX48804
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetospA
  • SizePrice
Western Blot of Rabbit anti-p35 antibody. Lane 1: p35 recombinant protein 50 ng load. Lane 2: p35 recombinant protein 100 ng load. Lane 3: p35 recombinant protein 250 ng load. Lane 4: p35 recombinant protein 500 ng load. Primary antibody: p35 antibody at 1:1,000 for overnight at 4oC. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for p35.
Product group Antibodies
GeneTex
Borrelia burgdorferi p35 antibodyGTX48805
ApplicationsWestern Blot, ELISA
ReactivityBacteria
  • SizePrice
Western blot showing detection of 0.1 μg of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein (arrow; expected MW: 63.1 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
Borrelia burgdorferi Osp C antibodyGTX48806
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityBacteria
TargetospC
  • SizePrice
Western blot showing detection of 0.1 μg recombinant proteins in Western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein (arrow; 59.5 kDa expected MW). Lane 3: fusion protein (MBP-tagged) plus cleaved fusion proteins (without MBP). Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in lane 3 are fusion protein (top band), or breakdown products of the fusion protein (bands in middle of blot). Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
Borrelia burgdorferi Erpn/Ospe antibodyGTX48807
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargeterpA
  • SizePrice
Western Blot showing detection of 0.1 μg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpD/Arp37 fusion proteins (arrow: expected MW of major band: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
Borrelia burgdorferi Erpd/Arp37 antibodyGTX48808
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetBB_RS05695
  • SizePrice
Western blot using GeneTex's Protein-A Purified anti-bovine VEGF antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa. Approximately 2 μg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA diluted 1:10. The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. Other detection systems will yield similar results.
Product group Antibodies
References
GeneTex
VEGFA antibodyGTX48811
ApplicationsWestern Blot, ELISA
ReactivityBovine
TargetVEGFA
  • SizePrice
Western blot using GeneTex's protein-A purified anti-pig CCL3L1 antibody shows detection of recombinant pig CCL3L1 at 7.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA, and probed with GeneTex's anti-pig CCL3L1 antibody. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
Product group Antibodies
GeneTex
CCL3L1 antibodyGTX48812
ApplicationsWestern Blot, ELISA
ReactivityBovine, Porcine
TargetCCL3L1
  • SizePrice
Western blot using GeneTex's protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with GeneTex's anti-bovine CCL2 antibody . After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
Product group Antibodies
References
GeneTex
MCP1 / CCL2 antibodyGTX48813
ApplicationsWestern Blot, ELISA
ReactivityBovine, Mouse
TargetCCL2
  • SizePrice
Western blot using GeneTex's anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with GeneTex's anti-bovine IL-21 antibody. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
Product group Antibodies
GeneTex
IL21 antibodyGTX48814
ApplicationsWestern Blot, ELISA
ReactivityBovine, Equine, Porcine
TargetIL21
  • SizePrice
Western blot using GeneTex protein A purified anti-TMBIM1 antibody (GTX48815) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize overexpressed TMBIM1 but not endogenous protein. The membrane was probed with the primary antibody at a 1:1,000 dilution at 4o C, overnight.
Product group Antibodies
GeneTex
Tmbim1 antibodyGTX48815
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetTMBIM1
  • SizePrice
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