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Primary Antibodies

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Western blot using GeneTex's affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
Product group Antibodies
References
GeneTex
Histone H2A.vS137ph (phospho Ser137) antibodyGTX48733
ApplicationsElectron Microscopy, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
ReactivityDrosophila
TargetHis2Av
  • SizePrice
Western blot using GeneTex's Affinity Purified anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 μg, 0.3 μg, 0.1 μg and 0.05 μg of purified mouse Mre11 protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4oC. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
Mre11 antibodyGTX48735
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetMre11a
  • SizePrice
Western blot using GeneTex's Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 μg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
References
GeneTex
RIF1 antibodyGTX48737
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetRif1
  • SizePrice
WB analysis of human kidney tissue using GTX48739 l-Myc antibody at 2.5μg/ml.
Product group Antibodies
GeneTex
l-Myc antibody, InternalGTX48739
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMYCL
  • SizePrice
Western blot analysis is shown using GeneTex's Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 mm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye?800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
Swi6 antibodyGTX48740
ApplicationsWestern Blot, ELISA
ReactivityYeast
Targetswi6
  • SizePrice
Western blot using GeneTex's Affinity Purified anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates. The expected molecular weight for SLIT-1 is 168 kDa. Approximately 20 μg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa.
Product group Antibodies
GeneTex
SLIT1 antibodyGTX48742
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rat
TargetSlit1
  • SizePrice
Western blot using Affinity Purified anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head). Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 μg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.
Product group Antibodies
GeneTex
Ajuba antibodyGTX48743
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
TargetAJUBA
  • SizePrice
Western blot using GeneTex's affinity purified anti-Hus1B antibody shows detection of a 36kDa band corresponding to Hus1B in a HeLa cell lysate (arrowhead). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa.
Product group Antibodies
GeneTex
HUS1B antibodyGTX48745
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
TargetHUS1B
  • SizePrice
This product was assayed by western blotting against RFA2 containing cell lysates. Blot incubated for 1 hr at RT with affinity purified Rabbit anti-RFA2 pan reactive Lot 13229 at 1.0 μg/ml followed by 1:5000 dilution of IRDye800 sheep anti-Rabbit IgG for 1 hr at RT.
Product group Antibodies
GeneTex
Rfa2 (Pan Reactive) antibodyGTX48746
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityYeast
TargetRFA2
  • SizePrice
This product was assayed by western blotting against RFA2 containing cell lysates. Affinity purified rabbit anti-RFA2 pS122 specific Lot 13230 at 1.0 μg/ml for 1 hr at RT followed by IRDye 800 sheep anti-Rabbit IgG1:5000 1 hr RT.
Product group Antibodies
GeneTex
Rfa2 (phospho Ser122) antibodyGTX48747
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityYeast
TargetRFA2
  • SizePrice
Western blot using GeneTex's affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 μg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4oC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
LDB1 antibodyGTX48750
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Mouse
TargetLdb1
  • SizePrice
RAD23B antibody
Product group Antibodies
GeneTex
RAD23B antibodyGTX48751
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetRAD23B
  • SizePrice
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