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Primary Antibodies

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Western blot using GeneTex's protein-A purified Anti-MIP-1 beta (CCL4) antibody shows detection of recombinant pig MIP-1 beta (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 4 μg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.
Product group Antibodies
GeneTex
MIP1 beta antibodyGTX48752
ApplicationsWestern Blot, ELISA
ReactivityPorcine
TargetCCL4
  • SizePrice
Western blot using GeneTex's protein-A purified anti-pig TNFa antibody shows detection of recombinant pig TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 1 μg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
Product group Antibodies
GeneTex
TNF alpha antibodyGTX48753
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityPorcine
TargetTNF
  • SizePrice
Product group Antibodies
GeneTex
IL17F antibody (Biotin)GTX48755
ApplicationsDot Blot, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIl17f
  • SizePrice
Western Blot of Rabbit anti-IL-17F antibody.(HRP) Load: 50 ng Mouse IL-17F per lane.Predicted/Observed size: 16 kDa, 16 kDa for Mouse IL-17F. Other band(s): None.
Product group Antibodies
GeneTex
IL17F antibody (HRP)GTX48757
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIl17f
  • SizePrice
Western blot using GeneTex's anti-Mouse IL-17F antibody shows detection of a band ~18 kDa in size corresponding to recombinant mouse IL-17F. Recombinant mouse IL-17F was loaded on to an SDS-PAGE gel at 0.25 μg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4oC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reation with FemtoMax Chemiluminescent substrate. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
Product group Antibodies
GeneTex
IL17F antibodyGTX48759
ApplicationsDot Blot, Western Blot, ELISA
ReactivityMouse
TargetIl17f
  • SizePrice
Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 μg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Product group Antibodies
GeneTex
Esrp-1/2 antibody [23A7.C9]GTX48760
ApplicationsWestern Blot, ELISA
ReactivityMouse
  • SizePrice
Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 μg ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Product group Antibodies
GeneTex
ESRP1 antibody [27H12]GTX48761
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetEsrp1
  • SizePrice
Western blot using GeneTex's protein-A purified anti-bovine CXCL10 antibody shows detection of recombinant bovine CXCL10 at 9.3 kDa (arrowhead) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA, and probed with 1 μg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
Product group Antibodies
GeneTex
CXCL10 / IP10 antibodyGTX48762
ApplicationsWestern Blot, ELISA
ReactivityBovine, Goat, Sheep
TargetCXCL10
  • SizePrice
Western blot using GeneTex's Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA, and probed with 1 μg/mL primary antibody overnight at 4oC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
Product group Antibodies
GeneTex
IL1F5 antibodyGTX48763
ApplicationsWestern Blot, ELISA
ReactivityBovine
TargetIL36RN
  • SizePrice
GeneTex's anti-AHA1 monoclonal antibody was used at a 5-10 μg/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. Expression of AHA1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
Product group Antibodies
GeneTex
AHA-1 antibody [25F2.D9]GTX48775
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAhsa1
  • SizePrice
Western blot showing detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
Surface Lipoprotein P27 antibodyGTX48799
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetBB_RS05365
  • SizePrice
Western Blot showing detection of 0.1 μg of recombinant CRASP-2 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-2 fusion protein (arrow; expected MW = 67.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Product group Antibodies
GeneTex
CRASP-2 antibodyGTX48800
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetcspZ
  • SizePrice
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