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Western blot analysis of protein polyglutamylation by anti-Polyglutamate chain (polyE), pAb (IN105) (Prod. No. AG-25B-0030).Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the tubulin glut
Product group Antibodies
AdipoGen Life Sciences
anti-Polyglutamate chain [Poly-E], pAb (IN105)AG-25B-0030
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityAll Species
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-IL-36beta (human), pAb (IN107)AG-25B-0032
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-ANGPTL8 [Betatrophin] (human), pAb (IN108)AG-25B-0033
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Immunoblot analysis of protein glycylation using anti-Tubulin (glycylated), pAb (Gly-pep1) (Prod. No. AG-25B-0034). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the glycylases TTLL3 and TTLL8
Product group Antibodies
AdipoGen Life Sciences
anti-Tubulin (glycylated), pAb (Gly-pep1)AG-25B-0034
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityCanine, Human, Mouse
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-CD112R (mouse), pAb (IN109)AG-25B-0035
ApplicationsWestern Blot, ELISA
ReactivityMouse
  • SizePrice
anti-Gasdermin D (mouse), pAb (IN110)
Product group Antibodies
AdipoGen Life Sciences
anti-Gasdermin D (mouse), pAb (IN110)AG-25B-0036
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetGsdmd
  • SizePrice
anti-NLRC5 (mouse), pAb (IN113)
Product group Antibodies
AdipoGen Life Sciences
anti-NLRC5 (mouse), pAb (IN113)AG-25B-0038
ApplicationsWestern Blot
ReactivityMouse
TargetNlrc5
  • SizePrice
Figure 1: Immunoblot analysis of protein glutamylation with anti-beta-Tubulin (beta-monoE), pAb (IN115) (Prod. No. AG-25B-0039). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases and are run on a 10% SDS-PAGE (specific protocol to separate alpha- and beta-tubulin (Magiera & Janke, 2013)). Tubulin purified from pork brain was used as a positive control for glutamylated tubulin. The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the beta-monoE (1:5,000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In untransfected HEK-293T cell extracts, no tubulin is detected, even on high exposure. After expression of TTLL4, predominantly beta-tubulin is detected with beta-monoE, but the antibody also faintly detects the heterogeneous mixture of different, yet unidentified substrates modified by TTLL4. In cells expressing TTLL5 and TTLL6, the beta-monoE detects only beta-tubulin, while expression of TTLL7 generates beta-monoE-positive alpha- and beta-tubulin. In the purified brain tubulin, the antibody specifically detects glutamylation of beta-tubulin. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
Product group Antibodies
AdipoGen Life Sciences
anti-beta-Tubulin (beta-monoE), pAb (IN115)AG-25B-0039
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetTUBB2A
Figure 1: Detection of endogenous mouse GLUT1 in brain and liver (negative/low expression) membranes by immunoblotting using anti-GLUT1, pAb (IN116) (Prod. No. AG-25B-0040).
Product group Antibodies
AdipoGen Life Sciences
anti-GLUT1, pAb (IN116)AG-25B-0040
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSLC2A1
Figure 1: Detection of endogenous human GLUT2 in human liver membranes by immunoblotting using anti-GLUT2 (human), pAb (IN117) (Prod. No. AG-25B-0041).
Product group Antibodies
AdipoGen Life Sciences
anti-GLUT2 (human), pAb (IN117)AG-25B-0041
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetSLC2A2
Figure 1: Detection of endogenous mouse GLUT2 in mouse liver and brain (low expression control) membranes by immunoblotting using anti-GLUT2 (mouse), pAb (IN118) (Prod. No. AG-25B-0042).
Product group Antibodies
AdipoGen Life Sciences
anti-GLUT2 (mouse), pAb (IN118)AG-25B-0042
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityMouse
TargetSlc2a2
Figure 1: Detection of endogenous rat GLUT2 in rat liver membranes by immunoblotting using anti-GLUT2 (rat), pAb (IN119) (Prod. No. AG-25B-0043).
Product group Antibodies
AdipoGen Life Sciences
anti-GLUT2 (rat), pAb (IN119)AG-25B-0043
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityRat
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