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ProductsBack/ZO-1 antibody

ZO-1 antibody

GTX108592
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTJP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ZO-1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.19 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7082
  • Target name
    TJP1
  • Target description
    tight junction protein 1
  • Target synonyms
    ZO-1, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07157
  • Protein Name
    Tight junction protein 1
  • Scientific Description
    This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Han P, Lei Y, Liu J, et al. Cell adhesion molecule BVES functions as a suppressor of tumor cells extrusion in hepatocellular carcinoma metastasis. Cell Commun Signal. 2022,20(1):149. doi: 10.1186/s12964-022-00962-9
    Read this paper
  • Wei MF, Cheng CH, Wen SY, et al. Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects. Oxid Med Cell Longev. 2022,2022:7957255. doi: 10.1155/2022/7957255
    Read this paper
  • Fang PH, Lai YY, Chen CL, et al. Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions. Mol Med. 2022,28(1):71. doi: 10.1186/s10020-022-00499-0
    Read this paper
  • De Tomi E, Campagnari R, Orlandi E, et al. Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins. Int J Mol Sci. 2022,23(3). doi: 10.3390/ijms23031647
    Read this paper
  • Barbian ME, Owens JA, Naudin CR, et al. Butyrate supplementation to pregnant mice elicits cytoprotection against colonic injury in the offspring. Pediatr Res. 2022,92(1):125-134. doi: 10.1038/s41390-021-01767-1
    Read this paper
  • Watanabe D, Nakagawa S, Morofuji Y, et al. Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes. Pharmaceutics. 2021,13(9). doi: 10.3390/pharmaceutics13091484
    Read this paper
  • Kariya Y, Oyama M, Suzuki T, et al. αvβ3 Integrin induces partial EMT independent of TGF-β signaling. Commun Biol. 2021,4(1):490. doi: 10.1038/s42003-021-02003-6
    Read this paper
  • Fang W, Zhao P, Shen A, et al. Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells. Mol Med Rep. 2021,23(3):pii: 205. doi: 10.3892/mmr.2021.11844.
    Read this paper
  • Minamide K, Sato T, Nakanishi Y, et al. IRF2 maintains the stemness of colonic stem cells by limiting physiological stress from interferon. Sci Rep. 2020,10(1):14639. doi: 10.1038/s41598-020-71633-3
    Read this paper
  • Bisi S, Marchesi S, Rizvi A, et al. IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules. Nat Commun. 2020,11(1):3516. doi: 10.1038/s41467-020-17091-x
    Read this paper

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Figure 1. Western blot analysis of TJP1 using anti-TJP1 antibody (PB9234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody (Catalog # PB9234) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 185 kDa.
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