Anti-Integrin alpha V [17E6], Human IgG1, Fc Silent(tm), kappa

Absolute Antibody

Anti-Integrin alpha V [17E6], Human IgG1, Fc Silent(tm), kappa

9

The antibody reacts with extracellular domains on human alphav-integrin chains.

alphav-Integrin is an integral membrane protein involved in cell adhesion. Antibody screening by ELISA revealed the antibody reacted specifically with alphavbeta3. In ELISA on fixed cells (CELISA) the antibody showed a reaction pattern consistent with its recognition of the alphav integrin chain (cells expressing alphavbeta3 and alphavbeta5 (M21)). The ability of the antibody to recognize native alphav integrins was tested by FACS analysis, where the alphav-expressing cells (M21) reacted strongly with the antibody. Immunoprecipitation from surface labelled cells showed the antibody detected alphav integrin in M21 cells. Furthermore, alphav-integrins can function as receptors for vitronectin. However, the antibody had the ability to disrupt stable interaction between vitronectin and alphavbeta3, and blocked the growth of M21 tumors in nude mice.

Flow Cytometry, ImmunoPrecipitation, ELISA

Research Use Only

Monoclonal

17E6

ITGAV

integrin subunit alpha V

antigen identified by monoclonal antibody L230; CD51; integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; vitronectin receptor subunit alpha; VNRA; VTNR

Human

IgG1

Integrin alpha-V

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203