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Parkin (E3 Ligase) (human) (rec.) (His)
Product group Proteins / Signaling Molecules
South Bay Bio
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Michaelis-Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05microM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP15374
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0C6U8
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Michaelis-Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125microM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities a
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDQ96LD8
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDQ9P0U3
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0C6U8
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0C6U8
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Mdm2 E3 Ligase TR-FRET Kit
Product group Chemicals
South Bay Bio
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ITCH E3 Ligase TR-FRET Kit
Product group Chemicals
South Bay Bio
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Parkin E3 Ligase TR-FRET Kit
Product group Chemicals
South Bay Bio
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% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
Product group Chemicals
South Bay Bio
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% Signal to Background of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nM to 3.125nM mixed with UBA1, UBE2L3, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.
Product group Chemicals
South Bay Bio
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