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Primary Antibodies

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Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6,000 dilution.
Product group Antibodies
GeneTex
Trkct1 antibodyGTX48720
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetNtrk3
  • SizePrice
Western blot using GeneTex's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution.
Product group Antibodies
GeneTex
GRASP antibodyGTX48721
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetTamalin
  • SizePrice
WB analysis of human CLIC1 recombinant protein using GTX48722 CLIC1 antibody at 1:200.
Product group Antibodies
GeneTex
CLIC1 antibody, N-termGTX48722
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetCLIC1
  • SizePrice
Western blot using GeneTex's affinity purified anti-AHA1 antibody shows detection of AHA1 in Cos7 cells. For Lanes 2 and 4, Cos7 cells were transfected with pcDNA3-FLAG-AHA1. For Lanes 1 and 3, Cos7 cells were not transfected. Extracts (40 μg per lane) were electrophoresed and transferred to nitrocellulose. The membrane was probed with anti-AHA1 (lanes 1 and 2, 1:2,000 dilution) or anti-FLAG (lanes 3 and 4). The lower band seen in anti-AHA1 blotting (arrowhead) is endogenous AHA1.
Product group Antibodies
GeneTex
AHA-1 antibodyGTX48723
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetAHSA1
  • SizePrice
Western blot using GeneTex's affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).
Product group Antibodies
References
GeneTex
Cyclin E2 antibodyGTX48724
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetCcne2
  • SizePrice
Product group Antibodies
GeneTex
Aubergine/Sting antibodyGTX48725
ApplicationsWestern Blot, ELISA
ReactivityDrosophila
Targetaub
  • SizePrice
GeneTex's affinity purified anti-MLF1IP pT78 antibody was used at 20 μg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.? Tissue was formalin-fixed and paraffin embedded.? The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Product group Antibodies
GeneTex
Mlf1 Interacting Protein (phospho Thr78) antibodyGTX48727
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCENPU
  • SizePrice
Western blot using GeneTex's affinity purified Anti-MDM2 (Rabbit) is shown to detect a band (arrow) corresponding to mouse MDM2 protein present in mouse MEF cells (lane 2) but not human kidney HEK293 cells (lane 1). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4o with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
MDM2 antibodyGTX48728
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityMouse
TargetMdm2
  • SizePrice
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.
Product group Antibodies
GeneTex
Mer2 antibodyGTX48729
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityYeast
TargetREC107
  • SizePrice
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.
Product group Antibodies
GeneTex
Mer2 (phospho Ser30) antibodyGTX48730
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityYeast
TargetREC107
  • SizePrice
Western blot using GeneTex's affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP.
Product group Antibodies
GeneTex
Mlf1 Interacting Protein antibodyGTX48731
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Primate
TargetCENPU
  • SizePrice
Product group Antibodies
GeneTex
Ego1 antibodyGTX48732
ApplicationsWestern Blot, ELISA
ReactivityC. Elegans
Targetego-1
  • SizePrice
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